s473 (Cell Signaling Technology Inc)
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99
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Cell Signaling Technology Inc
s473
S473, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 32625 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/phospho+akt+ser473/Phospho-Akt+(Ser473)+Antibody/pmc12816905-99-32-35
Average 99 stars, based on 32625 article reviews
S473, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 32625 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/phospho+akt+ser473/Phospho-Akt+(Ser473)+Antibody/pmc12816905-99-32-35
Average 99 stars, based on 32625 article reviews
s473 - by Bioz Stars,
2026-10
99/100 stars
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Incubation:Article Title: Exercise training-induced extracellular miR-136-3p modulates glucose uptake and myogenesis through targeting of NRDC in human skeletal muscle Article Snippet: .. Membranes were incubated with primary antibodies directed to NRDC (sc-137199; Santa Cruz Biotechnology, Dallas, TX, USA), AMP-activated protein kinase α (AMPKα) (#2532; Cell Signaling Technology, Danvers, MA, USA), Akt substrate of 160 kDa (AS160) (#2670; Cell Signaling Technology), phospho-AS160 (#8730; Cell Signaling Technology), protein kinase B (AKT) (#9272; Cell Signaling Technology), Article Title: Delivery of Pleckstrin-Homology Domains Suppresses PI3K/Akt Signaling and Breast Cancer Metastasis. Article Snippet: .. All membranes were blocked for 2 h in 3% BSA in Tris Buffered Saline (TBS) with 0.1% Tween-20 (TBST), before overnight incubation at 4◦C with primary antibodies to obscurin-Ig58/59 (rabbit polyclonal; 0.5 μg/mL[4]), HSP90 (C45G5, rabbit monoclonal antibody, Cell Signaling Technology, Danvers, MA; #4877), Myc-Tag (71D10, rabbit monoclonal antibody; Cell Signaling Technology, Danvers, MA; #2278), pan-Akt (C67E7, rabbit monoclonal antibody; Cell Signaling Technology, Danvers, MA; #4691), GAPDH-71.1 (mouse monoclonal antibody; Sigma, St. Louis, MO; G8795), PI3K/p85 (19H8, rabbit monoclonal antibody; Cell Signaling Technology, Danvers, MA; #4257), phospho-PI3K/P85-Tyr458/p55-Tyr199 (rabbit monoclonal antibody; Cell Signaling Technology, Danvers, MA; #4228), phosphoAkt-Thr308 (C31E5E, rabbit monoclonal antibody; Cell Signaling Technology, Danvers, MA; #2965), Article Title: ARHGAP40 suppresses clear cell renal cell carcinoma progression and is associated with altered glutamine-related metabolism and redox balance Article Snippet: .. Membranes were blocked with 5% non-fat milk in TBST and incubated overnight at 4 °C with primary antibodies: β -actin (CST, 4970, 1:1000), ARHGAP40 (MyBioSource, MBS9215052, 1:1000), AKT (CST, 9272, 1:2000), Saline:Article Title: Delivery of Pleckstrin-Homology Domains Suppresses PI3K/Akt Signaling and Breast Cancer Metastasis. Article Snippet: .. All membranes were blocked for 2 h in 3% BSA in Tris Buffered Saline (TBS) with 0.1% Tween-20 (TBST), before overnight incubation at 4◦C with primary antibodies to obscurin-Ig58/59 (rabbit polyclonal; 0.5 μg/mL[4]), HSP90 (C45G5, rabbit monoclonal antibody, Cell Signaling Technology, Danvers, MA; #4877), Myc-Tag (71D10, rabbit monoclonal antibody; Cell Signaling Technology, Danvers, MA; #2278), pan-Akt (C67E7, rabbit monoclonal antibody; Cell Signaling Technology, Danvers, MA; #4691), GAPDH-71.1 (mouse monoclonal antibody; Sigma, St. Louis, MO; G8795), PI3K/p85 (19H8, rabbit monoclonal antibody; Cell Signaling Technology, Danvers, MA; #4257), phospho-PI3K/P85-Tyr458/p55-Tyr199 (rabbit monoclonal antibody; Cell Signaling Technology, Danvers, MA; #4228), phosphoAkt-Thr308 (C31E5E, rabbit monoclonal antibody; Cell Signaling Technology, Danvers, MA; #2965), other:Article Title: Picalm coordinates clathrin-mediated endocytosis and actin remodeling during myogenesis Article Snippet: Western Blot:Article Title: ERMP1 Exerts Tumor‐Suppressive Functions in KIRC by Inhibiting PI3K/AKT Signaling and Remodeling the Immune Microenvironment: A Pan‐Cancer Analysis Article Snippet: .. Primary antibodies and dilutions: ERMP1 (rabbit; Proteintech, 27224‐1‐AP; WB 1:2000; and IHC 1:100); Phospho‐Akt (Ser473) (rabbit; CST, 4060 T; and WB 1:2000); Akt (rabbit; Proteintech, 10176‐2‐AP; and WB 1:2000); Phospho‐PI3K (rabbit; Abmart, T40065S; and WB 1:2000); PI3K (rabbit; Abmart, T40064S; and WB 1:2000); N‐Cadherin (rabbit; Affinity, AF4039; and WB 1:2000); E‐Cadherin (rabbit; Affinity, AF0131; and WB 1:2000); MMP2 (rabbit; Proteintech, 10373‐2‐AP; and WB 1:2000); MMP9 (rabbit; Proteintech, 10375‐2‐AP; and WB 1:2000); Cyclin D1 (mouse; Proteintech, 60186‐1‐Ig; and WB 1:2000); CDK4 (rabbit; Proteintech, 11026‐1‐AP; and WB 1:2000); GAPDH (rabbit; Affinity, AF7021; and WB 1:2000). .. Immunohistochemistry:Article Title: ERMP1 Exerts Tumor‐Suppressive Functions in KIRC by Inhibiting PI3K/AKT Signaling and Remodeling the Immune Microenvironment: A Pan‐Cancer Analysis Article Snippet: .. Primary antibodies and dilutions: ERMP1 (rabbit; Proteintech, 27224‐1‐AP; WB 1:2000; and IHC 1:100); |
